At least 100 µg of protein is required (with a concentration of ≥ 1 µg/µL). 

Because the required amount and condition of the sample may vary, prior consultation is essential.

For cell, cell media, tissue, and serum/plasma, please ship the samples in a Styrofoam box with dry ice.

For gel samples, please pack the gel and ship it with ice packs, ensuring that the gel does not dry out or warm up during transport.

After lysis, we measure protein concentration using a BCA assay, and we measure again at the peptide stage after digestion.

In general, if you send samples in the forms listed below, the PASS Center will perform pre-processing suitable for mass spectrometry.

For profiling, the workflow is as follows:

❶ Lysis : Protein denaturation using urea and guanidine

❷ Reduction & Alkylation : DTT for reduction, IAA for alkylation

❸ Digestion : Enzymatic digestion using trypsin and Lys-C

❹ Labeling & Fractionation (for relative quantification)

   : TMT labeling, followed by fractionation (12 or 24 fractions) to reduce complexity

❺ LC-MS/MS analysis : 120 min/1 run

❻ Data analysis

Thermo Scientific Orbitrap Exploris 480  & Orbitrap Astral	Thermo Scientific HF-X	Sciex Triple Quad 6500+	Thermo Scientific Orbitrap Astral & Brucker timsTOF
Profiling, TPD, Exosome	Interactome, HCP, PRM, Lipidomics	Targeted Proteomics	Single-Cell  Proteomics

Depending on your experimental objective, we provide the following. Additional items can be discussed in advance if needed.

❶ A matrix (Excel file) containing qualitative or quantitative protein information

❷ A report (Word or PDF) including sample information, experimental methods, and summary of results

❸ A data report (HTML file)

❹ MS raw files, search results, and database (for publications, provided via cloud download)

- For standard analysis (deep profiling), the turnaround time is within 8 weeks after we receive the samples.

- Additional services such as pre-processing (depletion, PTM enrichment, etc.) require about 2 extra weeks.

- For MRM/PRM analysis, peptide synthesis is outsourced and takes about 4 additional weeks.

- The PASS Center always strives to deliver analysis results as quickly as possible.